Days: Thursday, April 4th Friday, April 5th Saturday, April 6th
View this program: with abstractssession overviewtalk overview
View this program: with abstractssession overviewtalk overview
"Engineering nitrogen fixation ability in an oxygenic photosynthetic organism"
Deng Liu1, Maitrayee Bhattacharyya1, Michelle Liberton1, Jingjie Yu1, Thomas Mueller3, Costas Maranas3, Tae Seok Moon2, Fuzhong Zhang2, Jon M. Jacobs4, Richard D. Smith4, David W. Koppenaal4, Louis A. Sherman5, Himadri B. Pakrasi1,*
1Department of Biology; 2Department of Energy, Environmental, and Chemical Engineering, Washington University, St. Louis, Missouri, USA; 3Department of Chemical Engineering, Pennsylvania State University, State College, Pennsylvania, USA; 4Pacific Northwest National Laboratory, Richland, Washington, USA; 5Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
Certain cyanobacterial strains are capable of atmospheric nitrogen fixation, a counter- intuitive task, since nitrogenase, the enzyme that fixes nitrogen, is rapidly inactivated in the presence of oxygen. To achieve this goal, unicellular cyanobacteria such as Cyanothece 51142 separate these two activities temporally - they photosynthesize during the day and fix nitrogen during nighttime. Phylogenetically, Cyanothece 51142 is closely related to Synechocystis 6803. However, the latter strain cannot fix nitrogen. Our objective is to use a systems biology knowledgebase, exemplified by detailed models of metabolism and regulation, along with advanced synthetic biology tools to endow Synechocystis 6803 cells with the ability to fix nitrogen. Cyanothece 51142 has one of the largest intact contiguous nif gene cluster found in nitrogen-fixing cyanobacteria. The entire 28 kb cluster has been successfully transferred to Synechocystis 6803 and has remained stable for over five years. Under appropriate environmental conditions, the transformants can fix nitrogen. Genome-scale metabolic models have been developed for both Cyanothece 51142 and Synechocystis 6803 and are being implemented to aid in the fine-tuning of nitrogen fixation ability in the engineered Synechocystis cells.
Supported by funding from National Science Foundation and Department of Energy.
09:00 | Effects of mechanically active substrates on HaCaT cell cultures. (abstract) |
09:20 | Fabrication and characterizations of hydrogel microspheres for sustained delivery of platelet-rich plasma for the treatment of osteoarthritis (abstract) |
09:40 | Incorporation of decellularized extracellular matrix in cryogels for muscle regeneration (abstract) |
10:00 | Using Mesenchymal Stem Cell Transfected with Thymidine Phosphorylase for Gene-directed Enzyme Prodrug Therapy (abstract) |
10:20 | Resorbable Radiopaque Microspheres for Catheter Embolization using Microfluidics (abstract) |
10:40 | MANIPULATING AIR-GAP ELECTROSPINNING TO CREATE POLYMER NANOFIBER-WRAPPED GLASS MICROFIBERS FOR REGENERATING CRITICAL SIZE BONE DEFECTS OF CORTICAL BONE (abstract) |
11:00 | Exploration of extracellular macromolecular crowding effects on protein diffusion and interactions via Fluorescence Correlation Spectroscopy (abstract) |
Mark Haidekker (University of Georgia, United States)
09:00 | Development Of A Near-Infrared Daratumumab-based Contrast Agent For Preclinical Imaging Of CD38 Expression In Multiple Myeloma (abstract) |
09:20 | White Blood Cells Analysis for Leukemia Classification Using Image Processing Techniques (abstract) |
09:40 | Investigation of fatty acid recognition and internalization process by two receptors on living cell surface using Surface Enhance Raman Spectroscopy (abstract) |
10:00 | Tracking optical thin film and coating properties during dissolution via Optical Tunneling Photoacoustic Spectroscopy (OTPAS) (abstract) |
10:20 | Burn Wound Imaging Hand-held Device Via Optoacoustic Computed Tomography (abstract) |
12:30 | Biology-Inspired Engineered Agricultural and Food Systems (abstract) |
12:50 | Cellulolytic activity of edaphic fungi native to Yaqui Valley, Mexico, for the production of fermentable sugars from wheat straw cellulose pulp. (abstract) |
13:10 | Improved Antimicrobial Thin-Films for Food Processing (abstract) |
Christine O'Brien (Washington University in St. Louis, United States)
12:30 | Structural Protein-Based Whispering Gallery Microresonators for Ultraviolet Sensing (abstract) |
12:50 | Selective Isolation of Listeria monocytogenes in a Microfluidic Device (abstract) |
13:10 | Laser carbonized biosensors for Listeria monocytogenes detection in large sample volumes via vacuum driven flow (abstract) |
13:30 | A Novel Biosensor for Methicillin-Resistant Staphylococcus aureus (abstract) |
13:50 | Development of a speculum-free optical probe for cervical assessment during pregnancy (abstract) |
14:10 | Raman spectroscopy detects biochemical-specific changes in the cervix during human pregnancy (abstract) |
14:30 | "Detection by Death”: A rapid and inexpensive way to rapidly detect viable organisms of interest in complex substrates (abstract) |
14:50 | Direct-from-Sputum Rapid Phenotypic Drug Susceptibility Test for Mycobacteria (abstract) |
Join us for a tour to the Bayer-Monsanto Research Park!
David Mendoza-Cozatl (University of Missouri, United States)
David Mendoza-Cozatl (University of Missouri, United States)
Jianfeng Zhou (University of Missouri, United States)
14:45 | Chlorophyll fluorescence imaging and image processing to determine canopy leaf coverage (abstract) |
15:05 | Detection of Plant Disease Chemical Markers using a Microfluidic Biosensor Device (abstract) |
15:25 | Fast and Low-Cost Chlorophyll Fluorometer -- Second Iteration (abstract) |
15:45 | Adoption of Unmanned Aerial System (UAS)-based imaging system in Soybean Breeding Program (abstract) |
16:05 | Discrimination of Soybean Leaf Wilting due to Drought Stress Using UAV-Based imaging (abstract) |
14:45 | Hybrid Hyperthermia Strategy Using Multifunctional Nanostructures (abstract) |
15:05 | Templated Macroporous Polyethylene Glycol Hydrogels for Spheroid and Aggregate Cell Culture (abstract) |
15:25 | Lysozyme Sorption Behavior of Pure-Silica Zeolite MFI Films (abstract) |
View this program: with abstractssession overviewtalk overview
"How will innovative methods in improving stress tolerance of plants change the face of agriculture, and who cares?"
Agricultural systems have experienced significant shifts in recent years from a supply-driven paradigm to an integrated social, economic, and environmental scheme. While global demand of healthy foods rises with population growth, there are serious concerns about the availability, affordability, accessibility, and accountability of healthy foods (particularly fresh vegetables and fruits) across our nation. This presentation will begin with sharing some insights of the most recent Farm Bill with respect to the U.S. farming activities, socio-economical characteristics of rural/urban agricultural profiles, and challenges and barriers for small farms in disadvantaged communities. More discussions will focus on identifying knowledge gaps of existing sustainable management strategies of farming, followed by envisioning the applications of bionanotechnology and innovative data-driven platforms such as using new materials to measure stress tolerance in plants to alleviate financial vulnerability and personal/family/community anxiety created by climate variations, human interventions, and ecological transitions.
Support for this keynote is provided by the NSF ERC Planning Grant: Engineering Research Center for Materials for Agriculture Resource Imaging Analytics at High Resolution (MARIAH).
09:00 | Framework for the Professional Formation of Biological Engineers (abstract) |
09:20 | Teaching Creativity to Bioengineers—and Everybody Else (abstract) |
09:40 | MIT's Living Machines: An Engineering Education Transformation (NEET) Thread (abstract) |
10:00 | Development of Synthetic Biology Kits for STEM Education (abstract) |
10:20 | Implementing a Pedagogy of Creativity into Summer Programming in an NSF REU Site (abstract) |
10:40 | A BioMakerSpace at MIT enables hands-on, student-directed exploration of biological engineering (abstract) |
09:00 | Use of stem-loop DNA probes in biosensors targeting Legionella pneumophila in drinking water (abstract) |
09:20 | Photonic Molecularly Imprinted Polymer Film for the Detection of Testosterone in Aqueous Samples (abstract) |
09:40 | Low-cost, facile, and rapid nanosensors for establishing a participatory monitoring program on mercury exposure in rural Colombia. (abstract) |
10:00 | Algalytic bacteria increase methane production during anaerobic digestion of algal biomass (abstract) |
10:20 | Increasing biomethane production with the addition of Rotating Algal Biofilm Reactor algae for energy recovery in wastewater treatment (abstract) |
This session will be an interactive luncheon (lunch included in the conference) to address concerns, challenges and best practices in developing diversity and inclusion in sustainable intensification of precision agriculture. Relevant terminology, awareness, and practices will be discussed. The dialogue will draw on comprehensive training at North Carolina Agricultural and Technical State University (NCA&T, an 1890 land grant that houses the largest Historically Black College/University, HBCU, agricultural school and graduates the largest number of African American undergraduate and graduate engineers) as well as personal and administrative experiences of Dr. Kathleen Liang, co-Director, Center for Environmental Farming Systems, national eXtension Community of Practice leader, and policy advisory board for National Market Maker eCommerce.
Many organizations around the country have established policies to support diversity and inclusion at work environment to support, accommodate, and celebrate intelligence across age, gender, race and ethnicity, religion, culture, education, income, and other characteristics. However, the discussion and understanding of diversity and inclusivity have not been broadly shared across agriculture systems due to social, economic, and political barriers. Dr. Liang has worked with both 1862 and 1890 Land Grant Institutions, a variety of stakeholders and grass-roots organizations, and particularly socially/economically disadvantaged populations. This interactive luncheon will be a great opportunity to share lessons learned, stimulate an engaged discussion with the audience, and exchange ideas how we could enhance and improve our knowledge and experiences in developing diversity and inclusion in sustainable intensification of precision agriculture.
Dr. Kathleen Liang is the W. K. Kellogg Distinguished Professor of Sustainable Agriculture and co-Director of the Center for Environmental Farming Systems at North Carolina Agricultural and Technical State University. Dr. Liang recently received the ‘Best Practice Award’ at the 2019 National Small Business Institute Annual Academic Conference
Keith Roper (University of Arkansas, United States)
12:15 | Artificial Intelligence in Healthcare (abstract) |
12:35 | The Unstoppable Joins the Immutable: The Impact of Big Data and Blockchain on Healthcare (abstract) |
12:55 | Big Data: Reaping What We Sow (abstract) |
13:15 | Analyzing Big Data in Agribusinesses: Economical and Ethical Considerations in Health and Human Rights (abstract) |
13:35 | Transfer Printing of a Square Array of Gold Nanoparticles onto a Leaf Surface via Laser Induction and Resinous Adhesion (abstract) |
13:55 | Adoption of Unmanned Aerial System (UAS)-based imaging system in Soybean Breeding Program (abstract) |
12:30 | Enhanced production of indigoidine using a cold-shock inducible promoter (abstract) |
12:50 | Electricity Generation by Genetically Engineered Hyperthermophiles (abstract) |
13:10 | Characterization of the truncated caffeine N-demethylase reductase NdmD isolated from Pseudomonas putida CBB5 (abstract) |
13:30 | Regulating metabolic pathways and microbial subpopulations for chemical and biofuel production (abstract) |
13:50 | Bio-halogenation of flavonoids by a fungal flavin-dependent halogenase (abstract) |
14:10 | Sustainable production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (abstract) |
Silviya Zustiak (Saint Louis University, United States)
14:45 | Designing new nano-biocomposite materials using CNTs and ZnO hybrid interfaces and hydrogel environments for biomedical applications (abstract) |
15:05 | Microbial Growth Response to Highly Water Dispersible Boron Nitride Nanotubes (abstract) |
15:25 | Carbon-nanotube-polyethylene glycol nanocomposite biomaterials as neural substrates (abstract) |
15:45 | Optoelectronic plant leaf sensors with nanoantenna (abstract) |
16:05 | A Simple Microfluidic Device for the Production of Monodisperse Hydrogel Microspheres (abstract) |
16:25 | Interaction between nano-particle covered carbon nano-tubes and human blood (abstract) |
Ferris Pfeiffer (University of Missouri, United States)
15:30 | Mechanical Properities of atria and annulus in raidal and circumferential direction (abstract) |
15:50 | Effect of Stress Relaxation Time on Parameter Optimization Using Finite Element Simulation (abstract) |
16:10 | Design and Fabrication of a Novel Ultrasound Bioreactor (abstract) |
17:00 | Potential strategy to increase the quantum yield of photochemistry via the control of a redox switch in an antenna protein (abstract) |
17:00 | Struvite formation influenced by microalgae biofilms (abstract) |
17:00 | Analysis of Productivity and Characterization of Microalgae Grown on Bioreactor for Wastewater Remediation (abstract) |
17:00 | A Smartphone, Paper-Based Optical Platform for Whole Blood Glucose Colorimetric Assay (abstract) |
17:00 | Emerging Optical Materials for Whispering Gallery Mode Microresonators (abstract) |
17:00 | Design of a High Frequency Photo-Magnetic Actuator for Biomedical Applications (abstract) |
17:00 | Multiplexed Sensing by Two Microtoroid Cavities (abstract) |
17:00 | Investigation of Electrical Characteristics of Nanoporous GaN for Optoelectronic Neural Recording Probe (abstract) |
17:00 | Wastewater Remediation of Anaerobic Digester Effluent by a Rotating Algal Biofilm Reactor (abstract) |
17:00 | Surgical Needle Counter (abstract) |
17:00 | Production of Bioproducts from Waste Nutrients (abstract) |
17:00 | A novel, visual indicator for influenza detection (abstract) |
17:00 | Prolonged sitting affects somatosensory-evoked N300 and N500 (abstract) |
17:00 | Machine learning and eye tracking on motion-evoked EEG signals (abstract) |
17:00 | Microfluidic Cassette for Aerobic Growth of Microorganisms (abstract) |
17:00 | Machine Learning and Eye Tracking on Motion-Evoked EEG Signals (abstract) |
17:00 | Robotics Localization and Separation for Selective Auditory Attention in Hearing Aids (abstract) |
17:00 | Pediatric Wheelchair Assistive Device (abstract) |
17:00 | ORGANIC DERMAL INTRATISSULAR RAHABILITATION SYSTEM (abstract) |