Antimicrobial and Antibiofilm Study of D-Amino acidsCombinated with Nanoparticles Against Candida Albicans

EasyChair Preprint no. 2666

Abstract

Several studies reported possible antimicrobial activities of selected D-amino acids against Candida albicans. The present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibitory effects of certain D-amino acids, including D-glycine(D-gly) and D-aspartic acid(D-asp). Our findings indicate that D- aspartic acid is the most potent antifungal, among the examined D-amino acids, followed by D-glycine.Also The aim of this study was to evaluate the of D-amino acids and nanoparticles against Candida albicans adhered cells and biofilms. The results showed that the value of MIC for D-glycine was 50 μg /ml by well diffusion methods while in TCP methods the value of the MIC 100 μg/ml, MIC for zinc were 50 μg / ml for both methods. The effect of amino acid (D-glycine and D-aspartic acid) and nanoparticles (silver and lithium) in preventing the adhesion of cells on the surface of the polystyrene and the inhibition of the mature biofilm was studied. The results showed that the highest inhibition was at a concentration 50 μg /ml of D-asp, while less inhibition was given for the concentration 100 μg /ml of D-gly against cell adhesion and mature biofilm. The results proved that the combination of amino acid (D-glycine and D-aspartic acid) and nanoparticles (silver and lithium) at sub MIC concentrations (p≤0.05) inhibited cell adhesion and mature biofilm.

Keyphrases: antifungal, Antimicrobial, D-aminoacids

@Booklet{EasyChair:2666,
  author = {Saif T. Jasim and Shadman Tariq Sadiq and Ahmed Adel Abdelfatah Hamza},
  title = {Antimicrobial and Antibiofilm Study of D-Amino acidsCombinated with Nanoparticles Against Candida Albicans},
  howpublished = {EasyChair Preprint no. 2666},

  year = {EasyChair, 2020}}