Zeta potential for cell surface – nanoenvironment interaction assessment

EasyChair Preprint 127

Abstract

The pulsed electric field technologies are of great importance in biomedicine and biotechnology. While it is clear that at the physical level the electric field effects are mediated by charges and dipoles, the full mechanisms of electric field action on the cellular and biomolecular level are not understood in details. In this work, we attempt to understand the interaction of electrolyte ions with the cell surface in culture media and in different buffers. So far, we aligned our work with the buffers typically used in electroporation of cells. We used Zeta potential to assess cell surface-nanoenvironment interaction, where, the Zeta potential values in different buffers were different from the one measured in culture media. Therefore, the cell surface- nanoenvironment interaction has a prodigious influence on the final electric charge distribution, thus the subsequent phenomena caused by the pulsed electric field. It is also shown that Zeta potential characterizes this interaction taking into account the physicochemical properties of buffers and cell type simultaneously.

Keyphrases: Cell membrane surface charge, Zeta Potential Measurement, Zeta potential, cell membrane, conductivity buffer, electroporation, nanosecond pulsed electric field, pulsed electric field

@booklet{EasyChair:127,
  author    = {Djamel Eddine Chafai and Ingrida Nemogová and Pavel Dráber and Michal Cifra},
  title     = {Zeta potential for cell surface – nanoenvironment interaction assessment},
  doi       = {10.29007/zgpn},
  howpublished = {EasyChair Preprint 127},
  year      = {EasyChair, 2018}}