Strategy Propagation of Coffea Arabica L. by Tissue Culture Techniques

EasyChair Preprint no. 9455

Abstract

The research of in vitro callus induction on arabica coffe (Coffea arabica L.) 2.4 D, BAP, and NAA, was worked out in Tadulako University's Biotechnology and Genetics Laboratory, Tissue Culture Unit, Department of Biology, Faculty of Mathematics and Natural Sciences. The purpose of this research was to develop a callus induction method for Coffea arabica L. in vitro propagation using a variety of mediums. A completely randomized Design (CRD) with 6 treatments and 3 replications was used in this experimental study. BAP 1 ppm + NAA 0.2 ppm, BAP 1.5 ppm + NAA 0.2 ppm, BAP 2 ppm + NAA 0.2 ppm, 2.4 D 1 ppm + BAP 0.2 ppm, 2, 4 D 1.5 ppm + BAP 0.2 ppm, and 2.4 D 2 ppm + BAP were the different treatments. 0.2 ppm in. Based on the results of this study, it can be determined that 2.4D 2 ppm + 0.2 ppm BAP with a 100% callus percentage is the results of a proper and effective course of treatment. With a crumb texture and a white callus appearance.

Keyphrases: 2 4 D, BAP, callus, Coffea, NAA

@Booklet{EasyChair:9455,
  author = {Rizka Amaliah R Maruka and Meryany Ananda and Eny Yuniati and Asri Pirade Paserang},
  title = {Strategy Propagation of Coffea Arabica L. by Tissue Culture Techniques},
  howpublished = {EasyChair Preprint no. 9455},

  year = {EasyChair, 2022}}