Integration of Full-Thickness Human Skin and Autologous Immune Cells in Humanized Mouse and Rat Models

EasyChair Preprint no. 12558

Abstract

The utilization of animal models in biomedical research has significantly contributed to our comprehension of human physiology, disease mechanisms, and therapeutic approaches. Nonetheless, the inherent dissimilarities between human and animal immune systems present challenges in accurately predicting human-specific responses to diseases and therapies. A substantial stride toward addressing this disparity is the development of humanized mouse and rat models, incorporating full-thickness human skin and autologous immune cells. This paper delves into the methodology, applications, and potential impact of these advanced models on preclinical research and drug discovery. Human skin, acting as the primary barrier against pathogen infiltration, holds particular importance in infectious disease research. Despite the effectiveness of rodent models in studying human-specific skin pathogens, successfully co-engrafting human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of innovative human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, which involve the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells.

Keyphrases: Autologous Immune Cells, Full-Thickness Human Skin, Humanized Mouse and Rat Models

@Booklet{EasyChair:12558,
  author = {Isabella Rossi},
  title = {Integration of Full-Thickness Human Skin and Autologous Immune Cells in Humanized Mouse and Rat Models},
  howpublished = {EasyChair Preprint no. 12558},

  year = {EasyChair, 2024}}