Isolation and Identification of Endophytic Fungi of Rauwolfia Serpentina (L.) Benth. Ex Kurz by DNA Barcoding

EasyChair Preprint 9900

Abstract

Endophyte fungi are getting importance now a days for alternative source of valuable therapeutic compounds during their growth with host plants. Reports are available for possible association of endophytic microorganisms including bacteria, actinomycetes and fungi in different known plants having medicinal properties. Rauwolfia serpentina is a well-known Indian medicinal plant used in Ayurveda for treatment of snakebite, feverish illness and mental illness from ancient time and known for its antimicrobial, anti- inflammatory, antioxidants, antiproliferative, anticancerous, anti-diuretics, antifibrillar, anti-arrhythmic, anti-cholinergic, anti-dysentery, anti-diarrheal and anti-hypotensive properties. In the present study, an attempt was made to isolate endophytic fungi from leaves and stem parts of healthy young plant of Rauwolfia serpentina under laboratory conditions. Altogether 05 isolates namely Aureobasidium melanogenum, Aureobasidium pullulans, Aureobasidium iranianum, Simplicillium obclavatum and Phoma herbarum were obtained and identified through 18 S r RNA gene sequencing analysis. Isolated fungi were identified by sequence-based DNA Barcoding by using ITS region. Endophytic fungal DNA have been submitted to NCBI, Gene Bank and obtained Accession number for reference for further work and its phylogenetic tree was constructed by using MEGA 5.2 Software.

Keyphrases: DNA barcoding, Endophytic fungi, ITS region, Rauwolfia serpentina, phylogenetic tree

@booklet{EasyChair:9900,
  author    = {Neetu Das and Tanushree Chatterjee},
  title     = {Isolation and Identification of Endophytic Fungi of Rauwolfia Serpentina (L.) Benth. Ex Kurz by DNA Barcoding},
  howpublished = {EasyChair Preprint 9900},
  year      = {EasyChair, 2023}}