Antixodant Activity of Black Soybean Extract (Glycine Soja) by the DPPH (1,1-Diphenyl-2-Pcrylhydrazyl) Method

EasyChair Preprint no. 4123

Abstract

Black soybeans (Glycine soja) is a potential plant as a source of antioxidants rich in flavonoids, isoflavones and anthocyanins. Because it was conducted research that aims to determine the antioxidant activity of black soybean (Glycine soja) extract. The extract was obtained by maceration using ethanol solvent 70%, methanol, methanol-water (9:1), and ethyl acetate. Antioxidant activity test using scavenging 1,1-diphenyl-2-picrylhydrazil (DPPH) method, where the absorbance is measured on λ = 517 nm by spectrophotometer. The results showed that black soybeans had antioxidant activity as showed on the IC₅₀ value obtained. IC₅₀ value of ethanol extract 70% was 2.99 mg/mL, methanol extract was 2.17 mg/mL, methanol-water extract (9:1) was 1.58 mg/mL, and ethyl acetate extract was 0.23 mg/mL. The results suggest that ethyl acetate extract has the best activity as an antioxidant, so effective as an alternative source of antioxidants that can be developed in the pharmaceutical field.

Keyphrases: Antioxidants, black soybeans, DPPH

@Booklet{EasyChair:4123,
  author = {Nurisyah Nurisyah and Elisabeth Natalia Barung and Alfrida Monica Salasa},
  title = {Antixodant Activity of Black Soybean Extract (Glycine Soja) by the DPPH (1,1-Diphenyl-2-Pcrylhydrazyl) Method},
  howpublished = {EasyChair Preprint no. 4123},

  year = {EasyChair, 2020}}