Molecular Detection and Genotyping of Giardia Lamblia in Diarrheic Patients Using Nested PCR and RFLP-PCR by Gdh Gene

Title:Molecular Detection and Genotyping of Giardia Lamblia in Diarrheic Patients Using Nested PCR and RFLP-PCR by Gdh Gene

Authors:Fatima Akram Abd Ali and Intisar Ghanim Abdulwahhab

Conference:Tmrees25

Tags:gdh gene, Giardia lamblia, nested PCR, PCR genotyping and RFLP-PCR

Abstract:

This study was conducted to detect Giardia lamblia parasite and identify its genotypes based on the gdh gene using nested PCR and RFLP techniques. 100 stool samples were collected from patients with diarrhea, aged between 1 month and 41 years, during the period from October 2024 to July 2025. Microscopic examination revealed that (36%) of the samples were infected with the parasite. At the molecular level, DNA extraction was successful in 83 samples, of which 61 showed a primary amplification of (605 bp), and 51 samples showed a secondary amplification of (520 bp). RFLP analysis using RsaI enzyme revealed the presence of genotypes A and B, in addition to mixed infections. The prevalence of type B was 50.98%, followed by type A (39.21%), while mixed infections constituted (9.8%).