This study examines the effect of laser-induced plasma nitriding of Ti6Al4V alloy in the presence of nitrogen gas to improve its surface properties for medical applications. Treatment was performed using an Nd:YAG laser at a frequency of 1 Hz and energies of 280 and 460 mJ. The results showed an increase in surface roughness and microscopic hardness (from 240 to 308 HV) and an improvement in wettability with increasing laser energy. Based on the results of hardness and wettability, the sample treated at an energy of 460 mJ was chosen as the best sample for conducting corrosion and bacterial activity tests, as the corrosion rate decreased from 2.936×10⁻² to 9.208×10⁻³ mmpy with a reduction rate of approximately 68.7%, and the diameter of the bacterial inhibition zone increased from 20 mm to 26 mm against bacteria Staphylococcus aureus. The results confirm that laser processing improves the mechanical and biological performance of the alloy for medical implant applications.