Electric Motorcycles (EMs) are proving themselves to be efficient, low-polluting vehicles and a worthy replacement for gasoline-powered vehicles in Vietnam. This paper aims to study and introduce the road test procedure for EMs riding in Ho Chi Minh, Vietnam. The performance characteristics and energy consumption efficiency of EMs are evaluated using various input values. To reach this aim, two electric motorcycle prototypes were investigated through mathematical modeling and road test. Mathematical formulas clearly define key variables of interest such as vehicle mass, rider mass, speed, and frontal area. These are recorded by monitoring and measuring equipment listed with a technical description. The procedure starts with preparation, and inspection and ends with data analysis. Finally, case study applications in Ho Chi Minh, Vietnam applied and evaluated the obtained results. Energy consumption is 157 - 265 Wh/10km with a 21 – 27 km/h speed profile and powertrain efficiency with the 27 km/h profile is 87%. The experimental results like manufacturer's specifications and research reports on EMs. The next development direction is to research and evaluates rider behavior which is an important factor affecting the performance of EMs.