Acinetobacter baumannii is an opportunistic pathogen that affects people who have low immunity, particularly those at burn or wound infections and those in intensive care units. A total of 100 specimens were taken from people with burn and wound infections from emergency rooms of hospitals in Baghdad, including Al-Imamain Al-Kadhimain Medical City, Pediatric Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Kindy Hospital, Specialized Burns Hospital, Paediatric Protection Teaching Hospital, Ghazi Al-Hariri Hospital for Specialized Surgery. All patients collected samples were of both sexes and in many different age brackets. Specimens collected from August 1 to November 1, 2025 included 30 A. baumannii isolates; 17 isolates (32.07%) of burn infection and 13 (27.65%) of wound. Isolates were identified using the VITEK 2 Compact System and determined for antimicrobial susceptibility. A. baumannii positive isolates were tested for the ability to form biofilms and were found to be all biofilm positive. This shows a linkage between OmpA and CsuE genes with the bacterium's ability to form biofilms. All (100%) of the A. baumannii isolates from burn/wound infections were found to be able to produce biofilms. Molecular characterization of bacterial isolates was conducted using PCR, subsequently detecting OmpA and CsuE gene expressions in the biofilms produced by the biofilm-forming isolates. In the current work, OmpA was found in 80% of the A. baumannii isolates (100% of the biofilm-producing isolates), and CsuE was identified in 67% of isolates (all biofilm-producing isolates). Tryptanthrin significantly decreased the expression levels of both OmpA and CsuE genes through real-time PCR and down-regulated gene expression.