This study aimed to diagnose Cryptosporidium parvum infection in children using the TRAPC1 and TRAPC2 genes. One hundred stool samples were collected from patients attending Tikrit Teaching Hospital between October 1, 2024, and March 30, 2025. Using modified Ziehl-Neelsen stain, Cryptosporidium parvum was detected in 38% of the samples, with higher infection rates in males (42%) than in females (31.5%). The highest prevalence was observed in the 3-5 year age group (44.4%), while the lowest was in the 13-15 year age group (21.4%). Seasonal variation in infection rates was observed, with the peak prevalence in January (56%) and the lowest rate in October (16.6%). DNA was extracted from 100 stool samples and the results showed the presence of a DNA band in 93 samples out of a total of 100 samples. Conventional PCR diagnosis was then performed using primers for both the TRAPC2 and TRAPC1 genes on 93 samples and the results showed that the number of samples positive for the TRAPC2 gene was 18 samples (19.4%), while the TRAPC1 gene gave a positive result in 26 samples (27.9%).