In the paper we investigate the role of smart specialisation in the revitalization of Ukrainian industrial sector through the prism of industry experts’ opinions. The opportunities of smart specialization implementation for industrial revitalization are considered; and the readiness for European standards of smart specialization implementation in the industrial enterprises is assessed. In Ukraine smart specialisation is only being mastered according to the results of Ukrainian industrial enterprises survey held in 2019 by authors. The key finding of the research is that representatives of Ukrainian industry though perceive the importance of this tool face many obstacles in its implementation. Considering research results the efficient solutions of identified problems were proposed. They include the establishing an effective dialogue between central and local authorities, national producers and academic institutions; development of new areas of activity through targeted forms of interregional cooperation; development and implementation of the industrial and financial policy tools to stimulate R&D and S3 implementation on regional level.