This paper highlights the results of the experimental verification of the pedagogical conditions efficacy to build tolerance among future officers of the National Guard of Ukraine (NGU). The identified pedagogical conditions to build the NGU future officers’ tolerance are changes to the educational environment, filling the latter with practical examples of the benefits of being tolerant; faculty development and course officers’ professional development in terms of building learners’ tolerance; identification of the educational components to form tolerance as a quality, corresponding changes to these components’ educational and methodological packages. The experiment has demonstrated that the learners of the mixed groups, both females and males, have higher levels of their tolerance in both CG and EG before and after the experiment. Therefore, the pedagogical conditions for the formation of the NGU future officers’ tolerance should also embrace, if possible, the formation of mixed training groups of future officers, including both males and females.