Introduction: In Brazil, there is an effort to incorporate interprofessional education (IPE) in policies that induce professional reorientation in health. The Education Program through Work for Health - Interprofessionality (PET-I) stands out, which applies the theoretical and methodological bases of the IPE to promote curricular changes for undergraduate courses in the health area, in addition to initiatives of permanent education. Objectives: To describe the experiences and knowledge collectively built in the implementation of PET-I in a Brazilian public university. Methods: Exploratory study carried out through documental analysis of portfolios and conducting two focus groups with PET-I participants (before and after interventions by PET-I). For analysis, content analysis was adopted. Results: From the analysis of the responses of the Focus Groups and of the portfolios, three categories emerged. This study chose to focus on the category: Interprofessionality and teamwork. In the first focus group, it was possible to identify speeches that evidenced the competition in the daily routine of the teams. After the experience in the PET-I, during the conduction of the second focus group, there was a maturation in the understanding of IPE, in view of the improvement of their skills with interprofessional work and the debate on the reality of health education. Conclusions: The activities sought to develop collaborative skills by giving autonomy to participants, encouraging protagonism for greater interprofessional collaboration. Therefore, it was necessary for the members to know and trust each other and communicate to develop a collective work, and the adoption of focus groups as a method was essential to guarantee the interaction between the participants. This experience was the great trigger for learning and reflection on comprehensive care and teamwork.