In the present study, the authors intend to examine the potential of gold nanoparticles and thyme extract as an anti-tick to explore the therapeutic efficacy of the two extracts in the treatment of ticks, gold nanoparticles (AuNPs) and thyme Thymus vulgaris extracts. And the research was conducted on 268 tick samples of cattle in different locations of Salah al-Din Governorate, Iraq. The citrate reduction method has been used to prepare gold nanoparticles and the diameter of the particle is about 20 nanometers. The activity of the various concentrations of gold nanoparticles (2, 3, 4, and 5 mg/ml) and thyme extract (12.5, 25, 50, and 100 mg/ml) on ticks was experimented and their effects were observed at various time intervals (ranging between 30 minutes and 24 hours). The findings indicated the presence of lethal activity of both gold nanoparticles and thyme extract on ticks, and the effectiveness of both extracts augmented with growing levels of concentration and exposure duration. The synergetic performance of gold nanoparticles and thyme extract was also tested and the findings indicated that this combination enhanced the performance of the treatment as compared to the performance of the extracts used separately. This two extracts combination enhanced the rate of action and kill rate. The principal findings of the given research are that gold nanoparticles and thyme extract may serve as the alternative to conventional chemical pesticides to eliminate ticks. Their synergistic interaction gives them a more potent effect and it is possible to suggest that such a combination can be used in the tick control methods in a more sustainable and environmentally-friendly way