Rheumatoid arthritis (RA) needs biomarkers to complement conventional serological markers while better reflecting immune activation and persistent inflammation Note that therefore the goal of this study was to compare relative expression levels of miR-155 and serum concentrations Galectin-3 suPAR CRP ESR among RF positive patients (RP) CCp=positive patients (AP) and healthy volunteers. Methods: A total of 90 subjects were recruited, with equal allocation between RF-positive rheumatoid arthritis (n=30), Anti-CCP-positive rheumatoid arthritis (n=30) and healthy control groups (Individuals age >18 years O.Cursors. The diagnosis was performed according to both the criteria from American College Rheumatology as well International League Against Rheumatism.) Galectin-3 and suPAR were assessed by enzyme-linked immunosorbent assay (ELISA) whereas relative miR-155 expression was determined using one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) with U6 as the endogenous reference, followed by analysis via 2−ΔΔCt method..