The aim of this study was to investigate the potential ethanol-methane coupling fermentation from food waste (FW). In the first stage FW was successfully utilized for ethanol generation, then in second stage wastewater of ethanol distillation was used as a substrate for methane fermentation under optimum conditions of pH 7 and temperature 37°C. The anaerobic digestion effluent was reused for diluting FW in the later batch of ethanol fermentation. The stillage of ethanol production was completely digested within 20 days with maximum methane yield of about (569 ml/g VS). In addition, volatile fatty acids (VFAs) and alkalinity concentrations had no inhibition effect because of the stable pH range of (7 to 8.02) in the favoring of methane production. Ammonium-nitrogen concentration showed remarkable inhibition for methane production in the coupling system after 20 days of progress. This resulted in a decrease of methane yield. Interestingly, in spite of relatively lower ethanol production (37g/l), compared to conventional system using tap water (49.5g/l), the proposed coupling fermentation system revealed clear superiority and potential application.