This paper discusses the operation of the fatigue testing machine for cables and moorings, named MEA1000, which performs fatigue tests by bending of high-gauge steel cables under moderate tension. This machine has an exclusive and pioneering project in Latin America as it is the only one capable of conducting tests with cables up to 76 mm thickness. The machine is installed at the Petropolis Catholic University, in the Steel Cable Fatigue Testing Laboratory (LEMOC), and is the result of an agreement between Petrobras (National Petroleum Agency), Dom Cintra Foundation, and Petropolis Catholic University. The study analyzes the results obtained in the commissioning test of the MEA1000 machine to obtain information about its functioning, and present observations on the behavior of the steel cable and a critical analysis of the results and data on the cable performance and its reliability. As a result, through detailed observation of the graphs generated from the data obtained in the commissioning test, the behavior of the cable on the machine regarding its resistance to fatigue due to bending force and the contact with the tie components such as pulleys and lever hoist could be evaluated. Data on the behavior of the cable during the cycles, how long it took to break each wire and how long it took for the final break of the cable could be gathered and is a important source of information for the stakeholders involved in the mentioned agreement.