This research investigates the application of a surface plasmon resonance (SPR)-based biosensor for detecting hemoglobin concentration in blood, utilizing different Kretschmann configurations. Hemoglobin, a crucial protein in red blood cells, serves as a biomarker for various medical conditions. Its concentration directly influences blood refractive index, a parameter detectable by SPR biosensors. While gold and silver are the most commonly used metals for such biosensors, this paper explores the use of different metals and semiconductors as alternatives to the gold layer in the Kretschmann configuration. Specifically, the study examines the performance of Germanium, Gallium Arsenide, Aluminum, and Copper as the SPR layer and compares the results. To evaluate material performance, we conducted an angle sweep of the incident light source to determine the sensitivity of the SPR angle to refractive index changes. Simulations were conducted using the finite-difference-time-domain (FDTD) method, focusing on three parameters: sensitivity, full width at half maximum (FWHM), and quality factor to evaluate detection accuracy. The study found that copper is a highly effective replacement for silver and gold in hemoglobin detection. These findings offer valuable insights into the development of SPR based biosensors for medical diagnostics.