The hospital environment is considered a source of opportunistic infections so that infections related to hospital care can be acquired not only by patients but also by visitors and employees of the hospital itself. Thus, cleaning and disinfection of operating room surfaces between the end of a procedure and the beginning of another are essential to reduce the risk of dissemination of microorganisms. Objective: to quantify the load of organic matter on furniture and equipment surfaces in an operating room before and after cleaning and disinfection techniques. Method: this is an exploratory-descriptive study with a quantitative approach that investigated the load of organic matter through the bioluminescence of Adenosine Triphosphate (ATP) present on the surface of 08 pieces of equipment in a surgical room of a public hospital. For data analysis, absolute and relative frequencies were used, and with the application of the chi-square test, the results were presented in a graph and table. Results: it was revealed that 25% of the equipment showed satisfactory levels of ATP reduction, but only 12.5% of the items-maintained cleanliness levels. At the end of the study, ATP levels on surfaces were higher than estimated in 87.5% of the equipment. Conclusion: ATP levels were higher than estimated after the application of techniques to reduce organic matter, revealing inefficiency and indicating the need for continuous evaluation of the cleaning and disinfection processes of surfaces.

Keywords - Infection Control, Service, ATP, Organic Material, Operating Rooms.